Resident Evil: Apocalypse is set directly after the events of the first film, where Alice escaped from an underground facility overrun by zombies. She now bands together with other survivors to escape the zombie outbreak which has spread to the nearby Raccoon City. The film borrows elements from several games in the Resident Evil series, such as the characters Valentine and Oliveira and the villain Nemesis. Filming took place in Toronto locations including Toronto City Hall and Prince Edward Viaduct.
In the previous film, former security operative Alice and environmental activist Matt Addison fought to escape an underground genetic research facility called the Hive, the source of a zombie outbreak. The pair were part of an attempt to expose illegal experiments being performed thereby pharmaceutical company Umbrella Corporation. The film ended as Alice and Addison were taken into custody by Umbrella.
Umbrella sends a team to the Hive to investigate what happened there; the team is overrun by zombies who quickly spread to the nearby Raccoon City. Umbrella responds by quarantining the city and evacuating their personnel from it. Angela Ashford, the daughter of Umbrella researcher Dr. Charles Ashford, goes missing after her security car is involved in a collision while being extradited from the city. Meanwhile, disgraced Raccoon City Police Department Special Tactics And Rescue Squad (STARS) operative Jill Valentine returns to her former precinct to suggest her fellow officers that they evacuate Raccoon City. Alice awakens in a deserted hospital and wanders the city in search of supplies, while Umbrella uses the only bridge out of the area to evacuate civilians. At the bridge, Valentine meets with her former partner, Sgt. Payton Wells. A zombie bites and infects Wells. In response to the virus reaching the bridge, Major Timothy Cain, leader of the Umbrella forces in Raccoon City, seals the exit and forces the residents to return to the city.